Fig. 2.

Multiplex LF-IBBC protein assay from whole blood. (a) A representative fluorescence image of 8 barcodes each recording an identical panel of 11 proteins. Each barcode is bounded by a green fluorescent alignment marker. Each red bar records a unique protein; protein locations are determined relative to the alignment markers. The integrated fluorescence intensity of the 8 barcodes is presented below the fluorescent image, and reflects how reproducible barcode patterning leads to reproducible protein assays. (b) Fluorescence intensity of the first 5 biomarkers at each barcode repeat. (c) Correlation of the fluorescence intensity and corresponding protein concentrations for IL-8 and VEGF in logarithmic scale. The data reflect a better than 10% measurement error, except for the proteins present at the lower detection limits (<20 pg ml⁻¹). Eight whole barcodes were used to generate the mean fluorescence intensities and measurement uncertainties. The dashed lines are power law fits to guide the eyes.