Fig. 3.

Statistical comparison of 20 min and 40 min multiplexed protein assays using a LF-IBBC and pinpricks of whole blood from a single healthy donor. Mean fluorescence intensities are presented for each biomarker. Each measurement was performed in triplicate using 3 separate LF-IBBCs, but the same pinprick sample from a healthy donor. For each individual LF-IBBC assay, 8 barcodes were averaged to yield the measured protein levels. For this bar graph, the 3 separate LF-IBBC assays were then averaged. The error bars represent the measured standard deviation across the three separate LF-IBBC measurements. Differences between the 20 and 40 min LF-IBBC assays are calculated using the two-tailed Student’s t-test: (*), (**) and (***) indicate significant differences at P < 0.05, 0.01 and 0.001, respectively. The 40 min assay is clearly the most sensitive, but increasing assay times an additional 25–30% did not further increase sensitivity.