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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Cell. 2013 Apr 25;153(3):575–589. doi: 10.1016/j.cell.2013.03.024

Figure 5. Reduced expression of miRNAs and upregulation of their target genes inADAR1−/− mouse embryos at E11.5.

Figure 5

(A) Distribution of miRNA deep sequencing read lengths was compared between wildtype and ADAR1−/− mouse embryos at E11.0 and E11.5 stages. (B) Quantitation of select miRNAs expressed in E11.0 and E11.5 embryos. A spike control, 2 × 1010 copies of a chemically synthesized RNA, was added to the RT reaction mixture. Quantitative PCR was conducted in quadruplicate. The relative miRNA expression level standardized by the spiked RNA is presented as the expression level relative to that of the wildtype embryo at E11.0. Significant differences were identified by Student’s t-tests: *, p<0.05; **, p<0.01; ***, p<0.001 Error bars, S.D. (n = 5). (C) Increased protein expression of the genes targeted by the miRNAs reduced in ADAR1−/− embryos. Immunoblotting analysis of select target gene levels in E11.5 embryos (left) and a quantitative summary (right). Significant differences were identified by Student’s t-tests: **, p<0.01. Error bars, S.D. (n = 5).