(A) The domain structures of two ADAR1 isoforms, TRBP, and PACT. (B) Immunoblotting analysis of proteins involved in the pre-miRNA processing mechanism. Wildtype mouse embryos collected live at various embryonic stages (E9.0–12.0), and the extracted protein was fractionated by 4–15% SDS-PAGE and tested for immunoblotting analysis. (C) Quantitative summary of immunoblotting analysis results. The relative expression level was normalized by β-actin and presented as the expression level relative to that of the E9.0 embryo. Significant differences were identified by Student’s t-tests: *, p<0.05; ***, p<0.001. Error bars, S.D. (n = 4).