Table 3.
OTU diversity statistics for 95%, 97%, and 99% similarities.
| Identity (%) | # Seqs | OTUs | Goods coverage | Chao (CI) | Shannon (CI) | Shannon evenness |
|---|---|---|---|---|---|---|
| SUMMER | ||||||
| 99 | 450 | 52 | 0.900 | 382 (170–974) | 1.378 (1.20–1.55) | 0.349 |
| 97 | 450 | 17 | 0.976 | 45 (24–131) | 0.456 (0.33–0.58) | 0.161 |
| 95 | 450 | 9 | 0.984 | 30 (14–95) | 0.249 (0.16–0.34) | 0.113 |
| WINTER | ||||||
| 99 | 450 | 60 | 0.887 | 378.8 (182.1–891.9) | 1.46 (1.27–1.65) | 0.357 |
| 97 | 450 | 16 | 0.980 | 25 (18–56) | 0.44 (0.32–0.56) | 0.156 |
| 95 | 450 | 8 | 0.990 | 13 (9–40) | 0.24 (0.15–0.32) | 0.114 |
| WINTER CRUST | ||||||
| 99 | 323 | 55 | 0.860 | 303 (148–712) | 1.77 (1.55–1.99) | 0.442 |
| 97 | 323 | 20 | 0.960 | 46 (27–119) | 0.94 (0.78–1.11) | 0.316 |
| 95 | 323 | 16 | 0.900 | 34 (20–91) | 0.64 (0.49–0.79) | 0.230 |
CI, confidence interval. Summer and winter water samples normalized to the lowest sample effort (n = 450). The crust sample (LT37) is included for comparison but has lower sequencing effort than the grouped summer and winter samples.