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(A) Basophil degranulation: double-stained (CD 63, CD 203c) dot plot: from left to right unstimulated cells, positive control, stimulated with 10 μg/ml of dog saliva, dog dander extract and irrelevant allergen (rLep d 7), respectively. (B) Per cent upregulated basophils (y-axis) induced by serial dilutions of dog dander extract and saliva (x-axis); in blood from three representative dog-allergic patients.
