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. 2013 Mar 15;304(10):L665–L677. doi: 10.1152/ajplung.00370.2012

Fig. 5.

Fig. 5.

CYP1B1 compartmental mRNA and protein expression. RT-PCR: basal CYP1B1 mRNA was significantly higher in adults in both compartments, with the highest expression present in the airway (A), no exposure-related effects were observed in neonates (B), and adults had a time-dependent decrease in CYP1B1 expression in the airways at PFP days 24 (PFP24) and 48 (PFP48) (C). Data are presented as means + SE (n = 5–7 rats/group, in each compartment) *Significantly different compared with neonates in the same compartment. †Significantly different compared with airways in the same age. ‡Significantly different compared with FA in the same compartment. CYP1B1 immunohistochemistry showed a different spatial pattern. Whereas adults had more CYP1B1-positive cells basally, CYP1B1 was intensely upregulated in both ages. By PFP24, neonatal CYP1B1 began to fade from the most distal terminal bronchioles, whereas adult CYP1B1 expression remained elevated, as shown in the insets, as denoted by black arrows. At PFP48, neonatal CYP1B1 returned to FA control levels, whereas adults continue to remain slightly upregulated. Scale bar is 50 μm.