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. 2013 Apr 5;304(10):L701–L707. doi: 10.1152/ajplung.00259.2012

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Fig. 5. — Muc5ac mRNA expression, tissue inflammation index, and concentration of TNF-α in bronchoalveolar lavage (BAL) fluid. A: Muc5ac mRNA expression in homogenized lung tissue was determined by real-time PCR analysis and normalized to the expression of Gapdh. B: tissue inflammation, which was determined by hematoxylin and eosin staining, was not significantly different between the genotypes. Number of mice used for each condition: Tace^flox/flox/Cre^−/− with PBS (wild-type-PBS), n = 6; Tace^flox/flox/Cre^−/− with HNE (wild-type-HNE), n = 9; Tace^flox/flox/Cre^+/− with PBS (Tace conditional deletion-PBS), n = 3; Tace^flox/flox/Cre^+/− with HNE (Tace conditional deletion-HNE), n = 14. C: concentration of TNF-α in BAL fluid. Number of mice used for each condition: Tace^flox/flox/Cre^−/− with PBS (wild-type-PBS), n = 2; Tace^flox/flox/Cre^−/− with HNE (wild-type-HNE), n = 3; Tace^flox/flox/Cre^+/− with PBS (Tace conditional deletion-PBS), n = 3; Tace^flox/flox/Cre^+/− with HNE (Tace conditional deletion-HNE), n = 5.