Table 2.

I_Ca,L parameters in myocytes from sham-operated and MI hearts treated with pyruvate

	Untreated				Pyruvate
	n	Sham	n	MI	n	Sham	n	MI
ICa,L, pA/pF (0 mV)
Basal	33	−11.0 ± 0.4	10	−11.7 ± 0.3	7	−10.9 ± 0.6	10	−11.2 ± 0.4
Fsk, Δ	9	10.4 ± 1.7	8	1.6 ± 1.7*	6	10.7 ± 0.6	10	10.4 ± 0.5
Activation
V1/2, mV	29	−14.0 ± 1.1	8	−12.6 ± 1.6	9	−13.1 ± 1.2	5	−10.4 ± 1.6
k, mV	29	6.9 ± 0.1	8	6.6 ± 0.1	9	6.6 ± 0.7	5	6.3 ± 0.2
Inactivation kinetics
Tf , ms	29	7.3 ± 0.4	8	5.8 ± 0.4	9	6.0 ± 0.7	5	8.0 ± 0.9
Ts, ms	29	66.5 ± 3.4	8	65.3 ± 4.1	9	66.7 ± 2.6	5	73.2 ± 2.7

Values are means ± SE; n, number of myocytes. Myocytes were pretreated with 5 mmol/l pyruvate for 5–8 h before measuring L-type Ca²⁺ current (I_Ca,L) parameters. Δ, maximum change in current density, measured at 0 mV, in response to 1 μmol/l foskolin (Fsk); V_1/2, voltage at 50% activation; k, slope factor; T_f and T_s, fast and slow components of inactivation, respectively. Activation and inactivation kinetic parameters were measured at 0 mV.

^*P < 0.05 compared with sham.