A: Western blots of lipoma preferred partner (LPP), desmin, S6 ribosomal protein, and muscle LIM protein (MLP) in response to 48-h 5-mM l-NAME treatment [a nonspecific nitric oxide synthase (NOS) inhibitor], alone and in combination with mechanical stretch at 10% maximum strain at 1 Hz. Desmin (B), actin, S6 ribosomal protein (C), MLP (D), and LPP protein (E) expression in response to l-NAME and 10% mechanical stretch. *P < 0.05, compared with control; n = 4 cultures. F: Western blots of LPP, desmin, and S6 ribosomal protein in response to 48-h 5-mM l-NAME treatment (a nonspecific NOS inhibitor), alone and in combination with mechanical stretch at 20% maximum strain at 1 Hz. Desmin (G), actin, S6 ribosomal protein (H), and LPP protein (I) expression in response to l-NAME and 20% mechanical stretch. *P < 0.05 compared with control; n = 3 cultures. Samples were run in duplicate and averaged for each n of 1.