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Estrogen receptors (ERα and ERβ) bind to Egr-1 binding site in fetal hearts. Chromatin extracts prepared from 21-day fetal hearts were sonicated to produce DNA fragments between 100 and 500 bp in length. ERα and ERβ antibody-pulled chromatin extracts were used as templates for quantitative real-time PCR using primers flanking the element of Egr-1 binding site at PKCε promoter. M, markers. Data are presented as means ± SE; n = 5, analyzed by two-way ANOVA. *P < 0.05, female vs. male.
