Fig. 7.

TNF-α promoted the TRPV4 activation response to 4αPDD in PSC cultures from control rats. A: pretreatment of control PSC cultures with TNF-α (1 ng/ml) for 16 h produced a robust and sustained intracellular calcium mobilization in response to bath application of 4αPDD (30 μM) (top trace). The activity was sustained over 1 h with a single application of 4αPDD. In this experiment, 13 cells were activated from a total of 21 cells selected. In contrast, responses to 4αPDD were minimal for untreated control PSCs (bottom trace). B: histogram summarizes the averaged F340/F380 ratio to bath application of 4αPDD after TNF-α pretreatment (n = 21). Statistically significant increases in responses were observed for cells with TNF-α pretreatment compared with the untreated control group (n = 19, ***P < 0.001, Student's t-test).