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Semiquantitative immunoblotting analysis of CB2 in urothelium/suburothelium. Representive immunoblots are shown. The values determined by densitometry were normalized to those of GAPDH (loading control) in each sample. Exposure to acrolein for 3 h did not alter CB2 abundance in urothelium. Also, treatment with acrolein+GP1a or acrolein+AM630+GP1a did not affect abundance of CB2. n = 6 (saline-treated controls). n = 8 (other treatment groups).
