Fig. 4.

Semiquantitative immunoblotting analysis of phosphorylation of ERK1/2 (open bar), p38 (gray bar), and JNK (solid bar) in urothelium/suburothelium. Representive immunoblots are shown. Phosphorylation of ERK1/2 was increased 3 h after intravesical instillation of acrolein, and this effect of acrolein was attenuated by treatment with GP1a. The effect of GP1a was reversed by treatment with the selective antagonist AM630. n = 6 (saline-treated controls). n = 8 (other treatment groups). *P < 0.01 vs. saline-treated. #P < 0.05 vs. acrolein-treated. @P < 0.05 vs. acrolein+GP1a-treated.