Fig. 3.
Effect of alcohol consumption on total and phosphorylated mTOR (A), 4E-BP1 (B), and S6K1 in gastrocnemius of adult and aged female rats (C). Values are expressed as means ± SE; n = 8–9 rats per group. Bar graphs, quantitation of all Western blot data (D) for Ser2481-phosphorylated mTOR (autophosphorylation site), Thr37/46-phosphorylated 4E-BP1 and Thr389-phosphorylated S6K1 normalized to the total amount of the respective protein, and the control-fed value set at 100 arbitrary units (AU). C, control-fed; A, alcohol-fed. a,b,cValues with different letters are statistically different (P < 0.05), while those values with the same letter are not statistically different.