Skip to main content
. 2013 Mar 27;304(10):R887–R898. doi: 10.1152/ajpregu.00083.2013

Fig. 5.

Fig. 5.

Effect of alcohol consumption on binding of various proteins to raptor in gastrocnemius of adult and aged female rats. Values are expressed as means ± SE; n = 5–6 rats per group. Raptor was immunoprecipitated (IP) and then immunoblotted for mTOR (A), 4E-BP1 (B), Deptor (C), PRAS40 (D), and raptor (E). Quantitation of Western blot data were normalized to amount of raptor in IP, with the control-fed value for adult rats set at 100 AU. The black vertical line in the 4EBP-1 blot indicates a spliced figure, which was necessitated because the order of the adult and aged rats was inverted; however, all samples on this blot were run simultaneously on the same gel, just their order was reversed. a,b,cValues with different letters are statistically different (P < 0.05), while those values with the same letter are not statistically different.