Fig. 6.

Effect of alcohol consumption on total RagA and RagC, as well as the binding of RagA to raptor in gastrocnemius of adult and aged female rats. Top: Western blot for the total amount of RagA and RagC in whole muscle homogenate, where mTOR was used as a loading control. There were no age and/or alcohol effect on total RagA or RagC (n = 8 or 9 rats per group). Bottom: raptor was immunoprecipitated and RagA and raptor immunoblotted. Alcohol consumption, regardless of age, reduced binding of RagA to raptor (n = 4 per group).