Figure 1.
Heme induces ROS production by AM. Rat AMs (2 × 105/mL) were placed in a white flat-bottom 96-well plate. Cells were pretreated with DPI (10 μM) and apocynin (10 μM) and then stimulated or not with heme (1–30 μM) for 1 hour in the presence of luminol as a chemiluminogenic probe (500 mM). Absorbance intensity at 595 nm was directly proportional to ROS production by AM. DPI and apocynin alone had no effect. The data shown are representative of three independent experiments. The mean ± S.D. is presented. *P < 0.05 when compared with controls. **P < 0.05 when compared to cells treated with heme.