Figure 4.
Heme induces NO production by AM. (a) Rat AMs (1 × 106/mL) were pretreated with heme (10 μM) for 8 hours. Incubations were terminated by the addition of lysis buffer and lysates were subjected to immunoblotting with anti-iNOS, anti-arginase-1, and anti-tubulin antibodies. Results are demonstrated as the iNOS/Arg-1 ratio. Tubulin was used as a loading control. (b) Rat AMs (2 × 105/mL) cultured in 96-well plates were pretreated with heme (10 μM) for 24 hours. NO production was assessed by the Griess reaction and read on an Envision plate reader, as previously described [29]. The data shown are representative of three independent experiments. The mean ± S.D. is presented. *P < 0.05 when compared with controls.