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Heme induces NF-κB nuclear translocation in AM. Rat AMs (1 × 106/mL) were treated with heme (10 μM) for 1 hour. NF-κB nuclear content was assessed through immunocytochemistry using a Cy3-conjugated anti-p65 subunit antibody. LPS (1 μg/mL) was used as positive control. The data shown are representative of three independent experiments. Arrows: nuclei without NF-κB. Arrowheads: nuclei with NF-κB.
