Table 2.
Primers used in this study.
| Gene | Accession number | Forward primer and reverse primer | PCR product (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| White adipose tissue | ||||
| PPARγ | NM_013124 | F: CATGCTTGTGAAGGATGCAAG R: TTCTGAAACCGACAGTACTGACAT |
190 | 55 |
| Skeletal muscle | ||||
| Glut4 | M25482 | F: ACTGGCGCTTTCACTGAACT R: CGAGGCAAGGCTAGATTTTG |
106 | 56 |
| Liver | ||||
| PPARα | NM_011144 | F: ACCTCTGTTCATGTCAGACC R: ATAACCACAGACCAACCAAG |
352 | 55 |
| apoA-I | NM_009692.3 | F: ACATATATAGACCAGGGAAGAA R: AAACTGGGACACATAGTCTCT |
246 | 50.5 |
| apoC-III | NM_023114.3 | F: CAGTTTTATCCCTAGAAGCA R: TCTCACGACTCAATAGCTG |
349 | 47 |
| FAS | NM_007988 | F: TGGAAAGATAACTGGGTGAC R: TGCTGTCGTCTGTAGTCTTG |
240 | 50 |
| PEPCK | NM_011044.2 | F:CTACAACTTCGGCAAATACC R:TCCAGATACCTGTCGATCTC |
330 | 52 |
| SREBP1c | NM_011480 | F: GGCTGTTGTCTACCATAAGC R: AGGAAGAAACGTGTCAAGAA |
219 | 50 |
| SREBP2 | AF289715.2 | F: ATATCATTGAAAAGCGCTAC R: ATTTTCAAGTCCACATCACT |
256 | 47 |
| GAPDH | NM_031144 | F: TGTGTCCGTCGTGGATCTGA R: CCTGCTTCACCACCTTCTTGA |
99 | 55 |