Table 10.
Effects of aqueous rooibos extract, RPO, and/or their combination on glutathione status in the erythrocyte and liver of all experimental rats.
| Treatment | Erythrocyte | Liver | ||||
|---|---|---|---|---|---|---|
| GSH (µmol/µg protein) |
GSSG (µmol/µg protein) |
GSH:GSSG | GSH (µmol/g wet liver) |
GSSG (µmol/g wet liver) |
GSH:GSSG | |
| Negative control (water) | 0.210 ± 0.037 | 0.121 ± 0.012 | 1.70 ± 0.20 | 6.13 ± 0.09 | 0.46 ± 0.08 | 18.52 ± 1.57 |
| Positive control (t-BHP) | 0.064 ± 0.012* | 0.150 ± 0.014 | 0.41 ± 0.06* | 3.84 ± 0.39* | 0.56 ± 0.09 | 8.54 ± 1.63* |
| RTE | 0.190 ± 0.025 | 0.126 ± 0.011 | 1.59 ± 0.25 | 7.82 ± 0.47* | 0.23 ± 0.03* | 32.54 ± 4.35* |
| RPO | 0.195 ± 0.024 | 0.109 ± 0.005 | 1.75 ± 0.16 | 5.89 ± 0.39 | 0.23 ± 0.04* | 33.05 ± 6.48* |
| RTE + RPO | 0.321 ± 0.027* | 0.117 ± 0.003 | 2.72 ± 0.18* | 8.61 ± 0.52* | 0.22 ± 0.04* | 54.27 ± 10.35* |
| RTE + t-BHP | 0.159 ± 0.026# | 0.112 ± 0.005 | 1.42 ± 0.20# | 7.28 ± 0.52∗# | 0.32 ± 0.04# | 24.76 ± 2.96# |
| RPO + t-BHP | 0.182 ± 0.023# | 0.109 ± 0.004 | 1.65 ± 0.19# | 5.84 ± 0.29# | 0.14 ± 0.01# | 41.47 ± 2.58# |
| RTE + RPO + t-BHP | 0.240 ± 0.022# | 0.126 ± 0.008 | 1.89 ± 0.13# | 7.72 ± 0.27# | 0.37 ± 0.03# | 22.92 ± 3.24# |
Values are mean ± SEM of 8–10 rats per group. *Significantly different from negative control (P < 0.05). #Significantly different from positive control (P < 0.05). GSH: reduced glutathione, GSSG: oxidised glutathione, RTE: aqueous rooibos extract, RPO: red palm oil, t-BHP: tert-butyl hydroperoxide.