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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Surg Res. 2012 Sep 8;182(1):85–93. doi: 10.1016/j.jss.2012.08.053

Figure 1.

Figure 1

Knockdown of BRAF expression following transfection of siRNAin WRO human thyroid cancer cells. (A) Total RNA was quantified by qPCR at four different time points following transfection with 20 nmol of siRNA and compared to non-transfected cells and transfection with non-silencing siRNA. WRO cells exhibited a 51% (p<0.01) decrease in BRAF gene expression 24 hours post-transfection. This inhibitory effect was first observed at 12 hours and returned to near-baseline by 48 hours. (B) Western blot of P-ERK (a downstream actuator of the RAF/MEK/ERK signal pathway) showing a 27% reduction of protein levels when normalized to GAPDH 24 hours post-transfection and recovery of pre-transfection levels by 48 hours.