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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: Methods Mol Biol. 2011;720:173–181. doi: 10.1007/978-1-61779-034-8_10

Fig. 1.

Fig. 1

Schematic illustrating the coupled enzyme reactions that comprise the enzyme activity assay described in this chapter. First, spermine oxidase (SMO) catalyzes the FAD-dependent oxidation of spermine to spermidine, 3-aminopropanal, and H2O2. Second, in the presence of this H2O2, luminol is oxidized by horseradish peroxidase (HRP), resulting in production of chemiluminescence.