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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: Methods Mol Biol. 2011;720:173–181. doi: 10.1007/978-1-61779-034-8_10

Fig. 2.

Fig. 2

(a) Representative plot of H2O2 standard curve displaying H2O2 concentration (0–100 pmol in 300 μL reaction; x-axis) versus 103 RLU/min (y-axis). A best-fit line is also plotted (r 2 >0.99). (b) Representative data. A549 lung adenocarcinoma cells were treated for 24 h with or without 10 μM of the SMO-inducing polyamine analog, bis(ethyl)-norspermine (BENS). Cell lysate was harvested and SMO enzyme activity measured as described in this chapter.