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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: Methods Mol Biol. 2011;720:173–181. doi: 10.1007/978-1-61779-034-8_10

Fig. 3.

Fig. 3

Data generated using alterations to the described protocol, as described in Note 10. Purified, recombinant spermine oxidase protein was used with the current protocol for the determination of kinetic properties. Plot depicts Lineweaver–Burk transformation (r 2 >0.99) of SMO/PAOh1, assayed in the presence of increasing concentrations of spermine (0–250 μM).