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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Comp Neurol. 2012 Dec 15;520(18):4168–4183. doi: 10.1002/cne.23145

Figure 2. Co-localization of MC4R-GFP neurons with leptin-induced pSTAT3.

Figure 2

Double fluorescent IHC in ICV leptin-treated MC4R-GFP mouse brain sections revealed that leptin-induced pSTAT3 (magenta) and MC4R-GFP (green) are co-expressed in the mid-dorsal LHA (A). Digital zoom of boxed region in (A) is showed in (B). (C) Schematic drawing of distribution of pSTAT3 (magenta), MC4R-GFP (green) and double-positive (white) cells in 3 different levels of the LHA. Note that actual expression of NT and MC4R-GFP cells in other hypothalamic nuclei is not presented here. White arrows indicate representative co-localization. f, fornix; Scale bars: A, 80 um; B, 40 um.