Table 1.

Primary antibodies used in present study

Antigen	Immunogen	Manufacturer	Dilution used
GFP	Purified recombinant GFP emulsified in Freund’s adjuvant	Aves Labs (Tigard, OR), Catalog# GFP-1020, chicken polyclonal	1:1,000
NT	Synthetic peptide (human) Neurotensin (Glu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu) coupled to bovine thyroglobulin with glutaraldehyde	ImmunoStar (Hudson, WI), Catalog# 20072, Rabbit polyclonal	1:1,000
pSTAT3	Synthetic phosphopeptide of sequence ADPGSAAPyLKTKFIC corresponding to the amino acid residues flanking Tyr705 (lowercase y) of mouse STAT3	Cell Signaling (Danvers, MA), Catalog# 9131, Rabbit polyclonal	1:1,000
c-Fos	Synthetic peptide (SGFNADYEASSSRC) corresponding to amino acids 4–17 of human c-Fos	CALBIOCHEM (Gibbstown, NJ), Catalog# PC38, Rabbit polyclonal	1:10,000
FG	4-[(E)-2-(4-carbamimidoylphenyl)ethenyl]-3-hydroxybenzenecarboximidamide (Hydroxystilbamidine)	Chemicon, Catalog# AB153, Rabbit polyclonal	1:1,000
CTb	Cholera toxin subunit B (MIKLKFGVFFTVLLSSAYAHGTPQNITDLCAEYHNTQIHTLNDKIFSYTESLAGKREMAIITFKNGATFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMAN)	List Biological Laboratories, Catalog# 7032A6, Goat polyclonal	1:2,000
MCH	Asp-Phe-Asp-Met-Leu-Arg-Cys-Met-Leu-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Gln-Val	Phoenix Pharmaceuticals, Catalog# H-070-47, Rabbit polyclonal	1:10,000
Orexin	19 amino acids peptide mapping within amino acids 30–80 of human orexin	Santa Cruz Biotechnology, Catalog# sc-8070, Goat polyclonal	1:1,000
Nesfatin-1	VPIDVDKTKVHNVEPVESARIEPPDTGLYYDEYLKQVIEVLETDPHFREKLQKADIEEIRSGRLSQELDLVSHKVRTRLDEL	Phoenix Pharmaceuticals, Catalog# H-003-22, Rabbit polyclonal	1:1,000