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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Int Arch Allergy Immunol. 2012 Oct 16;160(3):251–258. doi: 10.1159/000341642

Figure 5. Restoration of CPE allergenic activity by reconstitution with Ara h 2 and Ara h 6.

Figure 5

Cells were sensitized with IgE from the serum pool (A, B) or with individual sera (C, D121; D, D123; E; D67). Sensitized cells were triggered with serial dilutions of CPE (●) or CPE w/o 20 kD (□) (A) or CPE (10 ng/ml) or CPE w/o 20 kD (10 ng/ml) plus either Ara h 2 (0.4 ng/ml) or Ara h 6 (0.6 ng/ml) or both as labeled (B–E). Values are expressed as per cent of maximal net degranulation seen with 200 ng/ml of CPE (set to 100%) for A and as percent of maximal net degranulation seen with 10 ng/ml of CPE (set to 100%) for B–E. In (A) the total degranulation with 200 ng/ml of CPE was 35–45% with < 10% background. The data are the average ± SEM of 4 separate experiments (A), 5 separate experiments (B), and 2 separate experiments (C–E).