Figure 3.

Lipopolysaccharide-induced increases in macrophage expression of proinflammatory factor mRNAs are restricted by cotreatment with oxidized galectin-1 in serum-free medium by reverse transcriptase polymerase chain reaction analysis. Reverse transcriptase polymerase chain reaction analysis: Relative levels of proinflammatory mRNA expressions A) IL-1β, B) IL-6, and C) iNOS after coapplication of lipopolysaccharide and oxidized galectin-1 in the serum-free medium. A) Average values of IL-1β mRN A intensity after combinatorial treatment (100L + 10G)/100L were 0.83 ± 0.26 (mean ± standard error of the mean) at two hours and 0.81 ± 0.08 at three hours post-treatment. Oxidized galectin-1 reduced the lipopolysaccharide-induced increase in IL-1β mRN A expression at three hours (^*t-test, P < 0.05, n = 6), but not two hours post-treatment. B) Average values of IL-6 mRNA intensity after combinatorial treatment (100L + 10G)/100LPS, were 0.85 ± 0.10 (mean ± standard error of the mean) at two hours and 0.70 ± 0.11 at three hours post-treatment. Oxidized galectin-1 reduced the lipopolysaccharideinduced increase in IL-6 mRNA expression at three hours (^*t-test, P < 0.05, n = 6), but not two hours post-treatment. C) Average values of iNOS mRN A intensity after combinatorial treatment (100L + 10G)/100LPS were 0.77 ± 0.17 (mean ± standard error of the mean) at two hours and 0.65 ± 0.13 at three hours post-treatment. Oxidized galectin-1 reduced the lipopolysaccharide-induced increase in iNOS mRNA expression at three hours (^*t-test, P < 0.05, n = 6), but not two hours post-treatment. 100L: 100 ng/mL lipopolysaccharide, 10G: 10 ng/mL oxidized galectin-1.

Abbreviations: iNOS, inducible nitric oxide synthase; Il-6, interleukin-6; IL-1β, interleukin 1β.