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(A) FACS analysis of the level of GFP in HepG2 cells transduced with the TGW and THIGW vectors. (B) Hamp, Hfe, GFP, and WPRE mRNA levels expressed by the TGW, THIGW, and THfeW vectors in HepG2 cells. Because the Hamp and Hfe sequences expressed by the vectors are originated from mouse, they were easily discriminated from the endogenous Hamp and Hfe sequences of HepG2 cells that are, instead, of human origin.
