Fig. 2.

Time course of protein expression of cdk1, a cell cycle checkpoint controller of S-to-G₂-to-M progression. (A) Western blot analyses of cdk1 expression from 0–to–72 h in the cytosolic or nuclear fractions prepared from control IHECs (Control), cells treated with BSO to maintain low GSH (Treated) or cells treated with BSO that was subsequently removed to allow for GSH recovery (Reversal). (B) shows the quantitative data of band intensities of cytosolic (closed circles) or nuclear (open circles) cdk1 expression relative to β-actin or H1, respectively for the 3 treatment groups. Results are mean±SEM for 4 separate blots. C illustrates the N-to-C ratios of cdk1. Statistical differences for time-group interactions among the 3 groups in (B and C) are denoted by letters a, b, c that are explained in the statistical analyses on the figure.