Fig. 3.

Kinetics of expression of nuclear chk2 and its phosphorylation status. (A) Western blot analyses of nuclear expression of chk2 and phospho-chk2 from 0–to–72 h in nuclear fractions prepared from control IHECs, cells treated with BSO to maintain low GSH (Treated) or cells treated with BSO that was subsequently removed to allow for GSH recovery (Reversal). (B) shows the quantitative data of band intensities of nuclear chk2 (closed circles) or phospho-chk2 (open circles) expression relative to H1 for the 3 treatment groups. Results are mean±SEM for 4 separate blots. (C) illustrates the N-to-C ratios of p-chk2-to-chk2. Statistical differences for time-group interactions among the 3 groups in (B and C) are denoted by letters a, c that are explained in the statistical analyses on the figure.