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A) Cells cultured on small (1,024 µm2) and large (10,000 µm2) islands differentiated into adipocytes and osteoblasts respectively after 1 week. Lipids stain red and alkaline phosphatase stains blue. Scale bar is 50 µm; B) Differentiation efficiency of hMSCs plated onto 1024, 2025, and 10,000 μm2 islands after 1 week of culture in mixed media without aphidicolin.
