Figure 3. Proliferation and apoptosis in developing Dlg5^−/− lung.

(A–D′) Immunofluorescent stainings of E12.5, E13.5 and E15.5 lung sections from wild-type (Ctrl) and Dlg5^−/− embryos with anti-BrdU (green in A–C′), anti-phospho-histone H3 (red, PH3 in A–B′, D–D′), anti-E-cadherin (epithelial cell marker, red in C–C′), anti-keratin 8 (epithelial cell marker, green, K8 in D–D′) antibodies. BrdU was injected into pregnant females 30 mins before euthanasia.

(E–F) Quantitation of the results shown in A–D′. Mitotic and proliferation indexes are determined as percentages of PH3+ and BrdU+ cells in the respective total cell population. Blue columns indicate epithelial and purple columns-mesenchymal cell types. Genotypes and ages are shown. Bars denote standard deviations, n=3–5 for each genotype.

(G–H′) Immunofluorescent stainings of E13.5 and E15.5 lung sections from wild-type (Ctrl) and Dlg5^−/− embryos with anti-E-cadherin (Ecad, red) antibodies and TUNEL staining for apoptotic cells (green).

(I) Quantitation of TUNEL stainings shown in G–H′. The graphs show percentages of apoptotic cells in the developing lung; ages and genotypes are shown. ** indicates P <0.05, t-test, n=4

Bar in A represents 0.2 mm in A–B′ and H–H′, 50 mm in C–D′ and 0.1 mm in G–G′.