Figure 3. Klebsiella pneumoniae induced inflammatory cytokines and chemokines in primary macrophages.
A) Thioglycolate-elicted peritoneal macrophages of indicated genotypes were infected with K. pneumoniae (MOI=200, 6 hours) or S. typhi (MOI=50, 1 hour). Cell free supernatants were analyzed on RayBiotech G Series 3 cytokine antibody arrays. Cytokines and chemokines induced ≥5 fold over the non-treated control of each genotype are shown. B) Quantification of IL-1β, MCP-1, and KC support trends observed on G Series 3 cytokine arrays. Cytokine levels were determined using RayBiotech Quantibody service.