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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: J Immunol. 2009 Jul 8;183(3):2008–2015. doi: 10.4049/jimmunol.0900138

Figure 6. NRLP3 regulated Klebsiella pneumoniae induced IL-1β and necrotic cell death in vivo.

Figure 6

A) Serum levels of IL-1β were significantly reduced in both Nlrp3 and Asc deficient mice challenged with K. pneumoniae p<0.05). B) A modest, yet significant, decrease in K. pneumoniae induced IL-1β was observed in the BALF of Nlrp3−/− mice compared to WT (p<0.05). In contrast, IL-6 levels were elevated in the serum (C) and BALF (D) of Nlrp3−/− mice following K pneumoniae infection. IL-1β and IL-6 were measured by ELISA. E) Decreased levels of cell death were detected in the BALF of K. pneumoniae infected Nlrp3 deficient mice as determined by LDH release. F) Serum levels of HMGB1 were dramatically reduced in mice lacking Nlrp3 as determined by western blot analysis. G) Cells obtained by BALF were collected from K. pneumoniae challenged WT and Nlrp3 deficient animals and were subjected to electron microscopy analysis. Cells from WT mice exhibited cell death features morphologically consistent with necrosis. In contrast, cells obtained from Nlrp3 deficient animals did not exhibit a morphology indicative of cell death. Magnified intracellular K. pneumoniae are shown in insets.