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. 2013 May 10;452(Pt 2):223–230. doi: 10.1042/BJ20130269

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Representative time course of RNA cleavage by SsoCas6 at 60°C under single-turnover conditions, analysed by gel electrophoresis and phosphorimaging. Labels ‘s’ and ‘p’ indicate substrates and products respectively. The control reaction ‘c’ was carried out in the absence of SsoCas6 for 25 min. (B) Single-turnover kinetic rates for cleavage of a CRISPR repeat RNA by SsoCas6 as a function of reaction temperature. The sequence of the RNA oligonucleotide substrate is shown at the top with the cleavage position indicated with an arrow. Each rate was calculated from at least six data points as described in the Experimental section, with means±S.E.M. calculated from curve fitting shown.