Table 1.

Summary of frequencies of alloreactive CTL-p obtained using different LDA protocols (11)

Responder cells	Stimulator cells	Filler cells	Maximum frequency	Target cells	Culture period (days)	IL-2 source
PBMC	PBMC	+	1:5000	PHA blasts	7	TCGF
PBMC/spleen	PBMC	+	1:870	PHA blasts	6	TCGF
PBMC	PBMC	+	1:733	PHA blasts	10	S
PBMC	PBMC	−	1:2000	PHA blasts	10	5 U/mL rIL-2
PBMC	PBMC/spleen	−	1:3322	PHA blasts	6	TCGF
PBMC	PBMC	−	1:646	B-LCL	10	2.5 U/mL riL-2
PBMC	B-LCL	−	1:900	B-LCL	10	TCGF
PBMC	T-cell clones	−	1:500	T-cell clones	10	5 ng/mL rIL-2
T cells	PBMC/spleen	−	1:240	ConA blasts	10	25 U/mL riL-2 + S
CD4+ T cells	PBMC	−	1:441	B-LCL	10	2.5 U/mL riL-2
CD4+ T cells	PBMC	−	1:1620	ConA blasts	10	25 U/mL riL-2 + S
CD4+ T cells	Spleen	−	1:11600	PHA blasts	14–18	TCGF
CD8+ T cells	Spleen	−	1:710	PHA blasts	14–18	TCGF
CD8+ T cells	PBMC	−	1:190	ConA blasts	10	25 U/mL riL-2 + S
T cells	Spleen	−	1:2010	PHA blasts	14–18	TCGF
T cells	B-LCL	−	1:66	B-LCL	10–12	25 U/mL riL-2 + S
T cells	B-LCL	−	1:535	ConA blasts	10–12	25 U/mL riL-2 + S

CTL-P, cytotoxic T-lymphocyte precursor; LDA, limiting dilution assays; PHA, phytohemagglutinin; TCGF, T-cell growth factor; S, MLC supernatant; B-LCL, EBV-transformed B-cell line; PBMC, peripheral blood mononuclear cells.