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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: N Engl J Med. 2010 Dec 22;364(7):627–637. doi: 10.1056/NEJMoa1006312

Figure 3. Effect of NFKBIA Expression in Patient-Derived Glioblastoma Cultures.

Figure 3

Primary cultures derived from three patients with glioblastoma are shown, each with a distinct NFKBIA and EGFR status (Patient 1: NFKBIA deleted [del, green font] and EGFR wild type [wt]; Patient 2: NFKBIA wt and EGFR amplified [amp, red font]; Patient 3: NFKBIA wt and EGFR wt). Cultures were infected with a retroviral vector expressing Flag-tagged NFKBIA (NFKBIA+). Protein lysates were subjected to immunoblotting for the detection of Flag-NFKBIA protein expression in relation to α-tubulin loading control. Mean cell viability, measured spectrophotometrically through bio-reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye by dehydrogenase enzymes of metabolically active cells, reveals a significant reduction in cell viability after expression of NFKBIA in NFKBIA-deleted tumor cells or in EGFR-amplified tumor cells, but no effect in tumor cells with wild-type status for both genes. P values were calculated with the use of an unpaired t-test. T bars indicate standard errors.