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. Author manuscript; available in PMC: 2013 May 13.
Published in final edited form as: Drug Metab Dispos. 2008 Mar 27;36(7):1212–1217. doi: 10.1124/dmd.107.019968

Fig. 3.

Fig. 3

Southern blot of DNA from rainbow trout hybridized with a 180-bp fragment of the rainbow trout hFMO cDNA. Total DNA from a single fish was isolated, and 5 μg was digested with the different restriction enzymes EcoRV, PstI, BamHI, EcoRI, and HindIII and double digestions HindII/PstI and BamHI/PstI; lanes 1 through 7, respectively. A 0.8% agarose gel was run and DNA blotted to a nylon membrane. Hybridization was carried out under high stringency conditions. Arrowheads indicate the predicted fragments generated from digestion of hFMO.