Figure 5.
Runx1 controls mechanosensitivity in VGLUT3+ C-LTMRs. A, photograph showing mechanical stimulation of a patch-clamped Tomato+ DRG neuron from ROSATomato/+; Vglut3Cre/+ control (Ctrl) mice. B, Families of rapidly adapting mechanosensitive current traces evoked by a series of mechanical steps in 2 μm increments for the DRG cells from Ctrl mice. C, Relationship between stimulation displacement and relative currents from B (n = 6). D, Representative traces of rapidly adapting, mixed adapting, and slowly adapting currents evoked by a short mechanical stimulus of 8 μm. E, Histograms showing the proportions of distinct mechanosensitive currents observed in small-diameter VGLUT3 neurons from control “Ctrl” (n = 4 mice, 51 neurons totally) and Runx1F/F;ROSATomato/+; Vglut3Cre/+ mutant (CKO) mice (n = 5 mice, 55 neurons totally), respectively. **p < 0.01, ***p < 0.001. Ctrl versus CKO, Student's unpaired t test. NS, Nonsignificant.