Table 1. Proteins significantly increased with OGD (18 hours).
| Uniprot accn no. | Gene name | Protein name | P value | Fold change | Protein function |
|---|---|---|---|---|---|
| Mitochondria | |||||
| P30048 | PRDX3 | Peroxiredoxin III | 0.0006 | 1.57 | Cell redox homeostasis |
| P61604 | HSPE1 | 10 kDa heat-shock protein | 6.1E-06 | 1.51 | Stress response |
| P18859 | ATP5J | ATP synthase-coupling factor 6 | 0.0016 | 1.44 | ETC complex V |
| O75489 | NDUFS3 | NADH-ubiquinone oxidoreductase 30 kDa subunit | 0.0030 | 1.38 | ETC complex I |
| P24752 | ACAT1 | Acetyl-CoA acetyltransferase | 9.1E-05 | 1.38 | Ketone body metabolism |
| P09622 | DLD | Dihydrolipoyl dehydrogenase | 0.0051 | 1.36 | Gycine cleavage |
| Q9UJZ1 | STOML2 | Stomatin-like protein 2 | 0.0052 | 1.36 | Receptor binding |
| P09972 | ALDOC | Fructose-bisphosphare aldolase C | 0.0061 | 1.34 | Glycolysis |
| P38606 | ATP6V1A | V-type proton ATPase catalytic subunit A | 0.0067 | 1.33 | ATP hydrolysis transport |
| P13804 | ETFA | Electron transfer flavoprotein subunit alpha | 0.0005 | 1.33 | Respiratory ETC |
| P30084 | ECHS1 | Enoyl-CoA hydratase | 0.0043 | 1.33 | Fatty acid metabolism |
| P40926 | MDH2 | Malate dehydrogenase | 0.0009 | 1.32 | Citric acid cycle |
| P10809 | HSPD1 | 60 kDa heat-shock protein | 0.0012 | 1.31 | Protein folding |
| P62258 | YWHAE | 14-3-3 protein epsilon | 0.0056 | 1.30 | Apoptosis |
| P04181 | OAT | Orthinine aminotransferase | 0.0012 | 1.30 | Amino acid biosynthesis |
| Q13011 | ECH1 | Delta (3,5)-delta(2,4)-dienoyl-CoA isomerase | 0.0048 | 1.28 | Lipid metabolism |
| Q99714 | HSD17B10 | 3-hydroxyacyl-CoA dehydrogenase type 2 | 0.0037 | 1.27 | Lipid metabolism |
| P07954 | FH | Fumarate hydratase | 0.0038 | 1.27 | Citric acid cycle |
| P30044 | PRDX5 | Peroxiredoxin 5 | 0.0014 | 1.26 | Redox homeostasis |
| P42765 | ACAA2 | 3-ketoacyl-CoA thiolase | 0.0067 | 1.26 | Lipid metabolism |
| P25325 | MPST | 3-mercaptopyruvate sulfurtransferase | 0.0012 | 1.25 | Response to Toxins |
| P30086 | PEBP1 | Phosphatidylethanolamine binding protein 1 | 0.0067 | 1.24 | Protease inhibition |
| P25705 | ATP5A1 | ATP synthase subunit alpha | 0.0019 | 1.21 | ETC complex V |
| Q06830 | PRDX1 | Peroxiredoxin 1 | 0.0009 | 1.20 | Redox homeostasis |
| P43686 | PSMC4 | 26S protease regulatory subunit 6B | 0.0007 | 1.20 | Protein degradation |
| Q16718 | NDUFA5 | NADH-ubiquinone oxidoreductase 13 kDa subunit | 0.0022 | 1.20 | ETC complex I |
| O00154 | ACOT7 | Cytosolic acyl-coenzyme A thioester hydrolase | 0.0009 | 1.19 | Acyl-CoA hydrolysis |
| Q00610 | CLTC | Clathrin heavy chain 1 | 4.9E-05 | 1.16 | Endocytosis |
| P12277 | CKB | Creatine Kinase B type | 0.0027 | 1.16 | Creatine metabolism |
| Endoplasmic reticulum | |||||
| O60568 | PLOD3 | Procollagen-lysine 2-oxoglutarate 5-dioxygenase 3 | 7.7E-05 | 1.87 | Lysyl hydroxylation |
| P27797 | CALR | Calreticulin | 1.0E-07 | 1.56 | Protein folding |
| P11021 | HSPA5 | Heat-shock protein 5 | 3.9E-05 | 1.53 | Protein complex assembly |
| P14625 | HSP90B1 | Heat-shock Protein 90 kDa beta member 1 | 5.5E-05 | 1.49 | Protein transport |
| P30101 | PDIA3 | Protein disulfide isomerase A3 | 2.7E-05 | 1.45 | Protein folding |
| Q16799 | RTN1 | Reticulon 1 | 0.0055 | 1.41 | Neuron differentiation |
| Q8NBS9 | TXNDC5 | Thioredoxin-domain containing protein 5 | 0.0017 | 1.40 | Cell redox homeostasis |
| P13667 | PDIA4 | Protein disulfide isomerase A4 | 1.4E-05 | 1.38 | Cell redox homeostasis |
| Q9Y2B0 | CNPY2 | Protein canopy homolog 2 | 0.0051 | 1.38 | Protein binding |
| Q15084 | PDIA6 | Protein disulfide isomerase A6 | 6.1E-06 | 1.37 | Chaperone |
| P54920 | NAPA | Alpha soluble NSF attachment protein | 0.0003 | 1.35 | ER-golgi vesicle transport |
| P14314 | PRKCSH | Glucosidase 2 subunit beta | 0.0016 | 1.33 | Glycoprotein formation |
| Q9NQC3 | RTN4 | Reticulon 4 | 0.0033 | 1.32 | Neurogenesis/apoptosis |
| Q14697 | GANAB | Neutral alpha-glucosidase AB/GNAB protein | 0.0018 | 1.27 | Glycoprotein cleavage |
| P07237 | P4HB | Protein disulfide isomerase | 0.0073 | 1.25 | Redox homeostasis |
| Q9BS26 | ERP44 | Endoplasmic reticulum resident protein 44 | 0.0007 | 1.23 | ER stress response |
| Q9NYU2 | UGGT1 | UDP glucose:glycoprotein glucosyltransferase 1 | 0.0074 | 1.23 | ER protein folding |
| P30040 | ERP29 | Endoplasmic reticulum resident protein 29 | 0.0003 | 1.23 | ER protein folding |
| P55072 | VCP | Valosin-containing protein | 0.0069 | 1.15 | ER stress response |
| Ribosomes | |||||
| P69905 | HBA1 | Hemoglobin subunit alpha | 0.0003 | 1.97 | O2 transport |
| Vesicle | |||||
| O15240 | VGF | Neurosecretory protein VGF | 0.0035 | 1.39 | Cell–cell interactions |
| P08133 | ANXA6 | Annexin 6 | 0.0075 | 1.18 | Ca2+ binding |
| P21281 | ATP6V1B2 | V-type proton ATPase subunit B | 0.0098 | 1.16 | H+ ion transport |
| P07900 | HSP90AA1 | Heat Shock Protein HSP90-alpha | 0.0030 | 1.11 | Stress response |
| Q9BTT0 | ANP32E | Acidic leucine-rich nuclear phosphoprotein 32E | 0.0034 | 1.08 | Phosphatase inhibitor |
| Cytoskeleton | |||||
| P52907 | CAPZA1 | F-actin capping protein subunit alpha 1 | 0.0039 | 1.35 | Protein complex assembly |
| P16949 | STMN1 | Stathmin | 0.0029 | 1.32 | Microtubule disassembly |
| Q9Y230 | RUVBL2 | RuvB like 2 | 0.0037 | 1.31 | Transcription |
| P47756 | CAPZB | F-actin-capping protein subunit beta | 3.2E-05 | 1.25 | Protein complex assembly |
| Q03252 | LMNB2 | Lamin B2 | 0.0076 | 1.24 | Cytoskeleton regulation |
| Q16352 | INA | Alpha-internexin | 4.9E-05 | 1.24 | Neurogenesis |
| P07437 | TUBB | Tubulin beta chain | 0.0016 | 1.23 | Microtubule constituent |
| P36405 | ARL3 | ADP-ribosylation factor-like protein 3 | 0.0071 | 1.23 | Cell division |
| P61163 | ACTR1A | Alpha centractin | 0.0029 | 1.23 | Vesicle mediated transport |
| Q9UJU6 | DBNL | Debrin-like protein | 0.0095 | 1.18 | Endocytosis |
| Q16658 | FSCN1 | Fascin | 0.0018 | 1.18 | Cytoskeleton organization |
| P62158 | CALM | Calmodulin | 0.0059 | 1.17 | Calcium modulation |
| Q9BZK7 | TBL1XR1 | F-box like/WD repeat containing protein TBL1XR1 | 0.0046 | 1.17 | Protein degradation |
| P49773 | HINT1 | Histidine triad nucleotide-binding protein 1 | 0.0002 | 1.15 | Signal transduction |
| P43487 | RANBP1 | Ran-specific GTPase-activating protein | 0.0029 | 1.15 | Signal transduction |
| Spliceosome | |||||
| Q07955 | SRSF1 | Serine/Arginine-rich splicing factor 1 | 1.1E-05 | 1.42 | mRNA splicing |
| Q01081 | U2AF1 | Splicing factor U2AF 35 kDa subunit | 0.0062 | 1.23 | mRNA splicing |
| Q15029 | EFTUD2 | 116 kDa U5 small nuclear ribonucleoprotein | 0.0069 | 1.19 | mRNA splicing |
| Proteosome | |||||
| Q15008 | PSMD6 | 26s proteasome non ATPase regulatory subunit 6 | 0.0053 | 1.61 | Protein degradation |
| P17980 | PSMC3 | 26S protease regulatory subunit 6A | 0.0071 | 1.27 | Protein degradation |
| Q13200 | PSMD2 | 26S proteasome non-ATPase regulatory subunit 2 | 0.0005 | 1.22 | Protein degradation |
| P54727 | RAD23B | UV excision protein RAD23 homolog B | 0.0009 | 1.22 | Protein degradation |
| Q99460 | PSMD1 | 26S proteasome non-ATPase regulatory subunit 1 | 0.0002 | 1.18 | Protein degradation |
| O00231 | PSMD11 | 26S proteasome non-ATPase regulatory subunit 11 | 0.0008 | 1.18 | Protein degradation |
| P28066 | PSMA5 | Proteasome subunit alpha type 5 | 0.0036 | 1.16 | Protein degradation |
ER, endoplasmic reticulum; ETC, electron transport chain; OGD, oxygen glucose deprivation.
Proteins are grouped according to subcellular compartment based on gene ontology and ranked according to magnitude of fold change. The significance threshold was set a priori at P<0.01.