Table 2. Proteins Significantly Decreased with OGD (18 hours).
| Uniprot accn no. | Gene name | Protein name | P-value | Fold change | Protein function |
|---|---|---|---|---|---|
| Mitochondria | |||||
| P21796 | VDAC1 | Voltage-dependent anion selective channel protein 1 | 0.0079 | 0.26 | Ion transport |
| P22695 | UQCRC2 | Cytochrome b-c1 complex subunit 2 | 0.0017 | 0.31 | ETC complex III |
| P31930 | UQCRC1 | Cytochrome b-c1 complex subunit 1 | 0.0097 | 0.46 | ETC complex III |
| Q9NS69 | TOM22 | Translocase of outer membrane 22 kDa subunit | 0.0027 | 0.46 | Protein transport |
| Q9Y3E5 | BIT1 | Peptidyl-tRNA hydrolase 2 | 0.0038 | 0.51 | Apoptosis |
| P02786 | TFRC | Transferrin receptor protein 1 | 0.0002 | 0.58 | Endocytosis |
| O75534 | CSDE1 | Cold shock domain containing protein E1 | 1.0E-06 | 0.62 | Transcription |
| Q9Y6H1 | CHCHD2 | Coiled-coil helix–coiled-coil helix domain protein 2 | 0.0099 | 0.70 | unknown |
| P11310 | ACADM | Medium chain specific acyl-CoA dehydrogenase | 0.0084 | 0.79 | Lipid metabolism |
| Endoplasmic reticulum | |||||
| Q81V08 | PLD3 | Phospholipase D3 | 0.0016 | 0.61 | Lipid degradation |
| Q96AG4 | LRRC59 | Leucine rich repeat-containing protein 59 | 0.0048 | 0.64 | ER membrane interactions |
| P13637 | ATP1A3 | Na+/K+ ATPase alpha (III) subunit | 0.0008 | 0.67 | ATP hydrolysis catalyst |
| P04843 | RPN1 | Ribophorin 1 | 0.0094 | 0.68 | Glycosyltransferase activity |
| Ribosomes | |||||
| P63173 | RPL38 | 60s ribosomal protein L38 | 0.0001 | 0.65 | Protein biosynthesis |
| P62249 | RPS16 | 40S ribosomal protein S16 | 7.9E-05 | 0.70 | Protein biosynthesis |
| P35544 | FAU | Ubiquitin-like protein FUB 1 | 0.0012 | 0.70 | Ubiquitination modulation |
| P62263 | RPS14 | 40S ribosomal protein S14 | 0.0004 | 0.74 | Protein biosynthesis |
| P62829 | RPL23 | 60S ribosomal protein L23 | 5.3E-05 | 0.74 | Protein biosynthesis |
| P62266 | RPS23 | 40S ribosomal protein S23 | 0.0004 | 0.76 | Protein biosynthesis |
| P46781 | RPS9 | 40S ribosomal protein S9 | 0.0002 | 0.78 | Protein biosynthesis |
| P08708 | RPS17 | 40S ribosomal protein S17 | 0.0004 | 0.80 | Protein biosynthesis |
| P42766 | RPL35 | 60S ribosomal protein L35 | 0.0018 | 0.82 | Protein biosynthesis |
| P26373 | RPL13 | 60S ribosomal protein L13 | 0.0004 | 0.84 | Protein biosynthesis |
| Vesicle | |||||
| P07355 | ANXA2 | Annexin A2 | 0.0038 | 0.49 | Ca2+ binding |
| P62491 | RAB11A | Ras-related protein Rab 11A | 0.0026 | 0.76 | Endocytic cycling |
| P07858 | CTSB | Cathepsin B | 0.0053 | 0.85 | Protein degradation |
| Cytoskeleton | |||||
| P06493 | CDK1 | Cyclin-dependent kinase 1 | 0.0022 | 0.57 | Cell-cycle control |
| P28289 | TMOD1 | Tropomodulin 1 | 0.0005 | 0.64 | Cytoskeleton organization |
| Q01518 | CAP1 | Adenylyl cyclase-associated protein 1 | 4.7E-06 | 0.70 | Signal transduction |
| Q9UQE7 | SMC3 | Structural maintenance of chromosomes protein 3 | 0.0084 | 0.71 | Cell cycle |
| P18206 | VCL | Viniculin | 0.0067 | 0.78 | Cell adhesion |
| Q01082 | SPTBN1 | Spectrin beta chain brain 1 | 0.0092 | 0.81 | Cytoskeleton movement |
| Spliceosome | |||||
| P17844 | DDX5 | Probable ATP-dependent RNA helicase DDX5 | 2.9E-06 | 0.58 | mRNA processing |
| Q9UKM9 | RALY | RNA-binding protein Raly | 0.0014 | 0.66 | mRNA splicing |
| Q86V81 | THOC4 | THO complex subunit 4 | 0.0007 | 0.75 | mRNA splicing |
| O43143 | DHX15 | Pre-mRNA splicing factor ATP-dep. RNA helicase | 5.5E-06 | 0.78 | mRNA splicing |
| P07910 | HNRNPC | Heterogeneous nuclear ribonucleoproteins Ca/C2 | 0.0005 | 0.81 | mRNA splicing |
ER, endoplasmic reticulum; ETC, electron transport chain; OGD, oxygen glucose deprivation.
Proteins are grouped according to subcellular compartment based on gene ontology and ranked according to magnitude of fold change. The significance threshold was set a priori at P<0.01.