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. 2013 Apr 27;13:93. doi: 10.1186/1471-2180-13-93

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Copyright © 2013 Ferreira et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bacterial DNase activity, treatment of the biofilm with DNase I and eDNA assay. Top: DNase activity was detected in culture supernatants of 16 ST1 isolates by measuring the halo size (cm) produced on Difco™ DNase Test Agar (BD). BU: Biofilm values for 16 ST1 isolates using inert polystyrene. Left bottom: For 16 ST1 isolates, 56U/well of DNase I were added to the culture media and the amount of biofilm accumulated determined. Right bottom: The concentration of eDNA determined in the biofilm supernatant. Isolate 08–008 (strong biofilm producer, agr-dysfunctional), 96/05 (moderate biofilm producer, agr-functional).