Figure 5. Syndecan-1 can be robustly shed by active matriptase.

HaCaT cells were incubated with increasing active matriptase, as indicated, at 37°C for 30 min. The conditioned media were collected and subjected to dot blot analysis with two different loaded volumes (1× and 2×) for syndecan-1. Data are representative of three independent experiments done under similar conditions.