Figure 1. Time course of the paralysis assay of CL4176 C. elegans.

Synchronized worms were hatched and fed on compound-containing NGM medium for two days at 16°C. The temperature was then shifted to 23°C. The survival rate (% of live worms) was recorded and plotted against the hours post temperature shift. The worms were treated with (A) galanthamine, (B) haemanthidine, (C) 1,2-Di-O-acetyllycorine and (D) 1-O-acetyllycorine at 10, 30 and 50 µM concentrations. In comparison, one group of worms were not treated (the non-treated control sample, CK) and the other group ware treated with 10 mM memantine. Three independent experiments were carried out to give the average rates for worm survival and to calculate standard deviations.