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. 2013 May 13;8(5):e63189. doi: 10.1371/journal.pone.0063189

Figure 5. MSK1 and MSK2 activity contribution to TPA-induced TFF1 expression.

Figure 5

A. Levels of MSK1 and MSK2 in transient MSK1 or MSK2 knockdown and scramble control (SC) MCF-7 cells were analyzed by immunoblotting. Actin was used as loading control. Note that MSK1 (7 µg, rabbit, Sigma) antibody was used at a higher titer than MSK2 (2.5 µg, rabbit, Invitrogen). B, C. Serum-starved transient MSK1 or MSK2 knockdown and scramble control MCF-7 cells were treated with TPA for 0, 15, 30, 45 and 60 min. Total RNA was isolated and quantified by real time RT-PCR. Fold change values, normalized to GAPDH expression levels and time 0 values, are the mean of three independent experiments, and the error bars represent the standard deviation. *P≤0.05 (Student’s paired t-test).