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. 2013 May 13;8(5):e63189. doi: 10.1371/journal.pone.0063189

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© 2013 Khan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MCF-7 cells were treated with scramble, MSK1, or MSK2 siRNA, serum starved for 72 h, and treated with TPA for 0 or 45 min. Protein lysates were analyzed by immunoblotting using antibodies against MSK1 (rabbit, Sigma), MSK2 (rabbit, Abcam ab99411), JUN, or FOS. Actin was used as a loading control.