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. 2013 May 13;8(5):e63863. doi: 10.1371/journal.pone.0063863

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© 2013 Kasmati et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Equal protein samples (20 µg) from 5-day-old homozygous, MS-grown plants of the indicated genotypes were analysed by immunoblotting using the antibodies shown. LHCP, light-harvesting chlorophyll-binding protein; OE33, oxygen-evolving complex 33 kD subunit; FNR, ferredoxin-NADP reductase; CPO, coproporphyrinogen oxidase; H3, histone H3. B,C. Chloroplast protein bands in A (as well as those from other, similar experiments) were quantified using Aida software (Raytest), and then the data were normalized using equivalent H3 data. Values shown are means (±SE) derived from 3–8 independent measurements.